Vybrant™ DiI Cell-Labeling Solution - Thermo Fisher Scientific
WEBDiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often …
Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine ...
WEBDiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange/red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often …
WEBInvitrogen offers a range of dialkylcarbocyanines with a variety of spectroscopic and cellular labeling properties: DiI (D282, D3911, N22880) and DiO (D275) have fluorescence excitation and emission maxima separated by about 65 nm, facilitating two-color labeling (Figure 1).1,4,10 DiO. Revised: 25–June–2008 | MP 00282.
WEBDiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling cell membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water.
WEBThe complementary Vybrant DiO and DiD cell-la-beling solutions allow cell populations to be marked in distinc-tive fluorescent colors for identification after mixing (Figure 1). Cells that have fused or formed stable clusters can be identified by double labeling (Figure 2).
Tracers for Membrane Labeling—Section 14.4 - Thermo Fisher Scientific
WEBThe Invitrogen™ Vybrant™ DiI cell-labeling solution is a dye-delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells.
WEBCells stained with CM-DiI or the sulfonated carbocyanines may be fixed by suspension in 3.7% (w/v) formaldehyde in PBS at 37°C for 10 minutes and permeabilized in acetone for 10 minutes at –20°C (Figure 1). Plasma membrane staining of PC12 rat pheochromocytoma cells by CM-DiI withstands fixa-
Direct labeling and visualization of blood vessels with lipophilic ...
WEBOct 9, 2008 · In the cell membrane, a DiI molecule has its two hydrocarbon chains buried in the lipid bilayer parallel to the phospholipid acyl chains, and the chromophores are on the surface of the bilayer...
DiOlistic labeling in fixed brain slices: phenotype, morphology and ...
WEBOur focus is on critical methodological components of DiOlistic labeling of cells in fixed slices, as well as protocols for combining this method with other staining protocols, e.g., immunolabeling of individual neurons (Alternate protocol 1).
CLARITY-compatible lipophilic dyes for electrode marking and …
WEBSep 6, 2016 · Fluorescent lipophilic dyes, such as DiI, stain cellular membranes and are used extensively for retrograde/anterograde labeling of neurons as well as for marking the position of extracellular...
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