Detection of Etoposide Resistance by Measuring DNA Damage in …
WEBMay 2, 1990 · The comet assay, which measures DNA strand breakage in individual cells, was used to examine the relation between DNA damage, cell survival, and resistance to the topoisomerase II inhibitor etoposide (VP-16).
Detection of etoposide resistance by measuring DNA damage in ... - PubMed
WEBMay 2, 1990 · The comet assay, which measures DNA strand breakage in individual cells, was used to examine the relation between DNA damage, cell survival, and resistance to the topoisomerase II inhibitor etoposide (VP-16).
Measuring DNA modifications with the comet assay: a …
WEBThe alkaline comet assay (single-cell gel electrophoresis) is a sensitive method that detects DNA strand breaks (SBs) and alkali-labile sites (ALS) in the nucleus of virtually all types of eukaryotic cells.
Comet assay: a versatile but complex tool in genotoxicity testing
WEBJan 5, 2021 · The comet assay is a versatile method for measuring DNA strand breaks in individual cells. It can also be applied to cells isolated from treated animals. In this review, we highlight advantages and limitations of this in vivo comet assay in a regulatory context.
Spheres of HeLa cells repair DNA damage caused by VP16
WEB(a) Representative fluorescence microscopy images of comet assays showing DNA damage caused by VP16 after 29 hours recovery. Short comet tail length, or its absence, indicates cells without...
Apoptosis of unstimulated human lymphocytes and DNA strand …
WEBEtoposide (VP-16)-induced DNA strand breaks and repair and apoptosis of unstimulated human lymphocytes have been studied using DNA comet assay, electrophoresis of low-molecular-weight DNA extracts, and fluorescence microscopy.
Measuring DNA modifications with the comet assay: a …
WEBJan 27, 2023 · The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human.
DNA double-strand breaks repair inhibitors potentiates the …
WEBJan 2, 2021 · NHEJ inhibitors sensitized human colorectal adenocarcinoma (LoVo) cells to VP-16, CDDP and VP-16/CDDP combination. CCK-8 assay was used to determine cytotoxicity of LoVo cells treated with DSBs inhibitors NU7441 and SCR7.
The comet assay: a method to measure DNA damage in individual …
WEBJun 27, 2006 · We present a procedure for the comet assay, a gel electrophoresis–based method that can be used to measure DNA damage in individual eukaryotic cells.
WEBComet assay exploits the ability of denatured or cleaved pieces of DNA to migrate out of the cell under the influence of applied electric current (electrophoresis). First cells are embedded into agarose gel at room temperature and are immobilized on comet slides.