Intrathecal Injections - Experimental Techniques - Pain Researcher
WEBDec 20, 2019 — The right volume for an intrathecal injection in mice is 5 ul, with a 5 ul chaser. Otherwise, you risk the injectate spilling over into the brainstem. The procedure is not easy because you have to get a feel of when the tip of the syringe is under the dura but still out of the spinal cord. The angle of the needle is crucial.
Intraganglionic injections into mouse DRG? - Pain Researcher
WEBJul 11, 2016 — But if you just want to hit DRG, it’s not necessary, unless you really just want a particular DRG (say, left L5 DRG). Intrathecal works very well, but it is dependent on titer and serotype and promoter. Many labs have found that AAV9 in the 10^13 vg/ml range (10 ul typical dose) works very well intrathecally.
Intrathecal siRNA injection for spinal neuron gene knockdown
WEBNov 3, 2016 — In the past we have used PEI to knockdown gene in both DRG and SC. Briefly, siRNA was dissolved in RNase-free water at the concentration of 1 μg/μl as stock solution, and mixed with polyethyleneimine (PEI, Fermentas Inc., Glen Burnie, MD), 10 min before injection, to increase cell membrane penetration.
Direct Intrathecal Injection of Recombinant Adeno-associated …
WEBMar 5, 2022 — Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult... Scientific Video Article | Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient...
Intrathecal catheter with port for multiple intrathecal deliveries in ...
WEBDec 8, 2017 — Hey all, I want to do repeated doses of drug to the mouse intrathecal space without needing to do an i.t. injection every time. i.t. injections are fine, but it’d be nice to already have the mice ready to go and just push a drug, perhaps multiple times. I know there are options to do chronic drug infusion with an Alzet pump, like this procedure from @anackley lab. But I’m looking for ...
Intraspinal injections - To Laminectomy or Not? - Pain Researcher
WEBJul 19, 2017 — The Kohro method avoids the damage of the laminectomy but really only allows a single injection, which could limit the spread of the virus to smaller area (L4 in their paper). Peirs et al ( @rpseal lab) used an approach on very P9-P21 animals using the non-laminectomy approach to good effect.
Syringes for Viral Injections? - Pain Researcher
WEBliz September 18, 2017, 7:35pm 2. We use the WPI UMP3 in our lab, which I’m assuming is just an older version of the UMP4, with a Hamilton syringe. Instead of using the glass pipette + Hamilton syringe combo, we simply use a small-volume (2.5ul) Hamilton syringe with a removable 28 or 33 gauge needle (also from Hamilton).
Death after intrathecal capsaicin? - Pain Researcher
WEBNov 16, 2023 — In the studies using systemic capsaicin injection in neonates to kill off the C-fibers (before TRPV1 was identified), it was common to see >50% mortality-the pups would turn blue and die. Sounds like it could be an interesting paper to characterize the sensory-sympathetic loop if anyone is willing to do the work.
EF1a promoter to drive expression in DRG in vivo?
WEBNov 17, 2017 — Experimental Techniques Reagents and Equipment. achamess November 17, 2017, 7:05pm 1. Has anyone used EF1a promoters to drive transgene expression in DRGs in vivo? I’ve had success with hSyn, and recently, a paper from Haenraets et al showed CAG works well for DRG. But EF1a is also very popular and it’d be nice to use.
Intraspinal injection of adeno-associated viruses into the adult …
WEBSep 4, 2021 — Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult. Experimental Techniques. 1: ...