OpenComet
WEBOpenComet is a plugin for the image processing program ImageJ. The latest version of OpenComet (v1.3) can be obtained from the Downloads page. Follow the instructions to install and launch OpenComet. OpenComet can analyze a batch of comet assay images, identify the comets and measure their parameters.
OpenComet v1.3 documentation - cometbio.org
WEBOpenComet is tested to perform well with various comet shapes and sizes including ones typical for neutral as well as alkaline comet assay. OpenComet works with a variety of magnifications, and has been tested for images captured between 5x to 40x magnification.
OpenComet: An automated tool for comet assay image analysis
WEBJan 9, 2014 · The comet assay is widely used for measuring oxidative DNA damage at a single cell level. The analysis of comet assay output images, however, poses considerable challenges. Commercial software is costly and restrictive, while free software generally requires laborious manual tagging of cells.
OpenComet | Benjamin M. Gyori, Ph.D. - Scholars at Harvard
WEBOpenComet can analyze a batch of comet assay images, identify the comets and measure their parameters. Refer to the usage instructions and a video tutorial to learn using the tool. Highlights. Automated analysis: faster and less prone to human bias. Robust methods for finding comets and comet head.
The Comet Assay: Automated Imaging Methods for Improved
WEBSep 1, 2016 · The comet assay, also referred to as single-cell gel electrophoresis (SCGE), has been extensively used in assessing genotoxic effects in cells exposed to various toxicants, including...
OpenComet: an automated tool for comet assay image analysis
WEBJan 9, 2014 · This paper presents OpenComet, an open-source software tool providing automated analysis of comet assay images. It uses a novel and robust method for finding comets based on geometric shape attributes and segmenting the comet heads through image intensity profile analysis.
CometAnalyser: A user-friendly, open-source deep-learning …
WEBAug 3, 2022 · Comet assay provides an easy solution to estimate DNA damage in single cells through microscopy assessment. It is widely used in the analysis of genotoxic damages induced by radiotherapy or chemotherapeutic agents.
OpenComet | BIII
WEBAutomatic detection of comet shapes with outliers detection. Automatic detection of the heads of comets (brightest region or profile analysis) Statistical values of tails, heads and Olive moments. Manual correction is available. Does live analysis with Micro-Manager.
The Comet Assay: Automated Imaging Methods for Improved …
WEBSep 1, 2016 · It is important to establish optimal parameters for image data collection before the comet assay can be used in routine studies. In this study we focus on the sources of variability in the imaging and analysis of comet assay samples using public domain and commercially available software.
OpenComet comet assay image analysis - GitHub
WEBOpenComet is an open-source software tool providing automated analysis of comet assay images. The open-access publication on OpenComet is available at BM Gyori, G Venkatachalam, PS Thiagarajan, D Hsu and MV Clement.
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