Comet assay - Wikipedia
WEBThe comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix.
Measuring DNA modifications with the comet assay: a …
WEBThe alkaline comet assay (single-cell gel electrophoresis) is a sensitive method that detects DNA strand breaks (SBs) and alkali-labile sites (ALS) in the nucleus of virtually all types of eukaryotic cells.
Measurement of DNA Damage Using the Neutral Comet Assay in …
WEBNov 11, 2021 · In this protocol, we describe in detail how to perform the neutral comet assay to assess double-strand breaks and their repair using cultured human cell lines.
Measuring DNA modifications with the comet assay: a …
WEBJan 27, 2023 · The neutral comet assay protocol developed by Olive et al. 28 to measure double SBs involves lysis in sodium dodecyl sulfate and incubation for 4 h at 50 °C with proteinase K—conditions ...
Evaluating In Vitro DNA Damage Using Comet Assay - PMC
WEBOct 11, 2017 · Quantitative assessment of DNA damage during experimental cancer therapy is a key step to justify the effectiveness of a genotoxic agent. In this study, we focus on a single cell electrophoresis assay, also known as the comet assay, which can quantify single and double-strand DNA breaks in vitro.
Minimum Information for Reporting on the Comet Assay (MIRCA ... - Nature
WEBOct 26, 2020 · The ‘neutral comet assay’ (whereby electrophoresis is carried out in a solution of neutral pH) is rarely used 22,23 and is therefore not included as a separate protocol step in the MIRCA ...
The comet assay: a method to measure DNA damage in individual …
WEBJun 27, 2006 · We present a procedure for the comet assay, a gel electrophoresis–based method that can be used to measure DNA damage in individual eukaryotic cells.
Measuring DNA modifications with the comet assay: a …
WEBThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated.
WEBThe Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. technique is based on micro-This electrophoresis of cells DNA content.
Neutral Comet Assay to Detect and Quantitate DNA Double …
WEBAug 20, 2021 · The comet assay is the most common and reliable method to study DNA strand breaks at the single-cell level. This procedure is based on the electrophoresis of agarose-embedded lysed cells. Following the electrophoretic mobilization of DNA, it is stained with fluorescent DNA-binding dye.
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